KANK1 Monoklonaler Antikörper

KANK1 Monoklonal Antikörper für WB, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

2D3G8

Kat-Nr. : 68613-1-Ig

Synonyme

KN motif and ankyrin repeat domain-containing protein 1, Kidney ankyrin repeat-containing protein, KIAA0172, KANK, Ankyrin repeat domain-containing protein 15

---

• 

  WB analysis using 68613-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68613-1-Ig (KANK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 68613-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using KANK1 antibody (68613-1-Ig, Clone: 2D3G8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of HepG2 using 68613-1-Ig

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug KANK1 Monoclonal antibody (68613-1-Ig, Clone:2D3G8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A375-Zellen, Daudi-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HuH-7-Zellen, MCF-7-Zellen, PC-3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

68613-1-Ig bindet in WB, IF/ICC, FC (Intra), ELISA KANK1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KANK1 fusion protein Ag31440
• Vollständiger Name: KN motif and ankyrin repeat domains 1
• Berechnetes Molekulargewicht: 147KD
• Beobachtetes Molekulargewicht: 130-200 kDa
• GenBank-Zugangsnummer: NM_015158
• Gene symbol: KANK1
• Gene ID (NCBI): 23189
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KANK1 (KN motif and ankyrin repeat domain-containing protein 1) is involved in the control of cytoskeleton formation by regulating actin polymerization, inhibiting actin fiber formation and cell migration (PMID: 25961457). It inhibits RhoA activity, the formation of lamellipodia but not of filopodia (PMID: 25961457, PMID: 5961457).It also inhibits fibronectin-mediated cell spreading and regulates Rac signaling pathways (PMID: 25961457). Talin-KANK1 interaction controls the recruitment of cortical microtubule stabilizing complexes to focal adhesions (PMID: 27410476). KANK1 can be detected 130-200 kDa (PMID: 15823577, PMID: 33253712, PMID: 35805114).