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KAP1 Rekombinanter Antikörper

KAP1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243043D10

Kat-Nr. : 85693-4-PBS

Synonyme

TRIM28, E3 SUMO-protein ligase TRIM28, EC:2.3.2.27, KAP 1, KAP-1

Filter:
  • Western Blot (WB) analysis of various lysates using KAP1 Recombinant antibody (85693-4-RR)

    WB analysis using 85693-4-RR (same clone as 85693-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85693-4-RR (KAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85693-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using KAP1 Recombinant antibody (85693-4-RR)

    IF Staining of HepG2 using 85693-4-RR (same clone as 85693-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KAP1 antibody (85693-4-RR, Clone: 243043D10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85693-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85693-4-RR

    Affinity and Kinetic Characterization of 85693-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85693-4-RR against Human KAP1 were performed. The affinity constant is 8.82 nM.

Geprüfte Anwendungen

Produktinformation

85693-4-PBS bindet in WB, IF/ICC, Indirect ELISA KAP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen KAP1 fusion protein Ag7350
Vollständiger Name tripartite motif-containing 28
Berechnetes Molekulargewicht 89 kDa
Beobachtetes Molekulargewicht100-120 kDa
GenBank-ZugangsnummerBC004978
Gene symbol KAP1
Gene ID (NCBI) 10155
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KAP1, also named as TRIM28 or RNF96, is a 835 amino acid protein, which contain one RING-type zinc finger, one PHD-type zinc finger, one bromo domain and two B box-type zinc fingers. KAP1 localizes in the nucleus and Belongs to the TRIM/RBCC family. KAP1 is a nuclear corepressor for KRAB domain-containing zinc finger proteins and mediates gene silencing by recruiting CHD3, a subunit of the nucleosome remodeling and deacetylation (NuRD) complex, and SETDB1 to the promoter regions of KRAB target genes. KAP1 is expressed in all tissues tested including spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes. The calcualted molecular weight of KAP1 is 89 kDa, but modified KAP1 is about 100 kDa. (PMID: 18590578 )