KATNA1 Polyklonaler Antikörper

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• KD/KO Validated

KATNA1 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

Kat-Nr. : 17560-1-AP

Synonyme

p60 katanin, Katanin p60 subunit A1, Katanin p60 ATPase-containing subunit A1, EC:5.6.1.1

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  WB analysis using 17560-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 17560-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17560-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17560-1-AP (KATNA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17560-1-AP

  IP result of anti-KATNA1 (IP:17560-1-AP, 4ug; Detection:17560-1-AP 1:500) with mouse brain tissue lysate 3440ug.

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  IHC staining of human bowen disease using 17560-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17560-1-AP (KATNA1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: human bowen disease; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IF: See 7 publications below

Produktinformation

17560-1-AP bindet in WB, IHC, IF, IP, ELISA KATNA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KATNA1 fusion protein Ag11753
• Vollständiger Name: katanin p60 (ATPase-containing) subunit A 1
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55-60 kDa
• GenBank-Zugangsnummer: BC050428
• Gene symbol: KATNA1
• Gene ID (NCBI): 11104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IF	Cell Res; Regulators of tubulin polyglutamylation control nuclear shape and cilium disassembly by balancing microtubule and actin assembly.; Authors - Lei Wang
human	WB	Nat Cell Biol; Microtubule minus-end regulation at spindle poles by an ASPM-katanin complex.; Authors - Kai Jiang
mouse	WB, IF	Neuron; Katanin p80 regulates human cortical development by limiting centriole and cilia number.; Authors - Wen F Hu
human	WB	Dev Cell; Microtubule Minus-End Stabilization by Polymerization-Driven CAMSAP Deposition.; Authors - Kai Jiang
mouse	IF	PLoS Biol; Primary cilium loss in mammalian cells occurs predominantly by whole-cilium shedding.; Authors - Mary Mirvis
human,rat	IF	Elife; Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission; Authors - Dawn M Wenzel