KATNAL1 Polyklonaler Antikörper

KATNAL1 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20166-1-AP

Synonyme

p60 katanin-like 1, p60 katanin like 1, Katanin p60 subunit A-like 1, katanin p60 subunit A like 1, Katanin p60 ATPase-containing subunit A-like 1

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  WB analysis of HEK-293 using 20166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20166-1-AP (KATNAL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 20166-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20166-1-AP (KATNAL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20166-1-AP

  IP result of anti-KATNAL1 (IP:20166-1-AP, 4ug; Detection:20166-1-AP 1:4000) with HeLa cells lysate 1280 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20166-1-AP bindet in WB, IP, ELISA KATNAL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KATNAL1 fusion protein Ag13960
• Vollständiger Name: katanin p60 subunit A-like 1
• Berechnetes Molekulargewicht: 490 aa, 55 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC000612
• Gene symbol: KATNAL1
• Gene ID (NCBI): 84056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KATNAL1 belongs to a family of microtubule-severing proteins and contains an N-terminal microtubule interaction and trafficking (MIT) domain, an ATPase AAA-type core domain, and a C-terminal Vps4_C domain. KATNAL1 is involved in regulating microtubule dynamics, which is essential for spermiogenesis and male fertility. It severs microtubules in an ATP-dependent manner, promoting rapid reorganization of cellular microtubule arrays(PMID: 22654668). In neurons, KATNAL1 plays a role in microtubule rearrangement during neuronal migration and process elongation. Additionally, mutations in KATNAL1 have been associated with intellectual disability and microcephaly in humans(PMID: 30448058).