KBTBD2 Monoklonaler Antikörper

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• KD/KO Validated

KBTBD2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A9F3

Kat-Nr. : 67895-1-PBS

Synonyme

BKLHD1, KBTBD2, KIAA1489

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  WB analysis using 67895-1-Ig (same clone as 67895-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67895-1-Ig (same clone as 67895-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67895-1-Ig (KBTBD2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67895-1-Ig (same clone as 67895-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KBTBD2 antibody (67895-1-Ig, Clone: 2A9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). DAPI (blue). This data was developed using the same antibody clone with 67895-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67895-1-PBS bindet in WB, IF/ICC, Indirect ELISA KBTBD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KBTBD2 fusion protein Ag4933
• Vollständiger Name: kelch repeat and BTB (POZ) domain containing 2
• Berechnetes Molekulargewicht: 622 aa, 71 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC047107
• Gene symbol: KBTBD2
• Gene ID (NCBI): 25948
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kelch repeat and BTB domain containing 2(KBTBD2), also known as BTB and kelch domain-containing protein 1, encodes a BTB-Kelch family subunit that p85α-specific recognition for the Cullin3-based E3 ubiqitin ligase complex, which regulates p85α protein ubiquitination and subsequently regulates phosphoinositide-3 kinases (PI3K) signaling (PMID:27708159). However, the expression profile and functional significance of Kbtbd2 is still largely unknown. The Human Protein Atlas which is an online source shows high Kbtbd2 expression in the spleen and thymus, suggesting that Kbtbd2 might involved in immunization (PMID:29331106). In addition, Kbtbd2 is related to Src-induced phosphorylation of protein, spermatogonial stem cell activity(PMID:15623525, 29331106).