KCNA3 Polyklonaler Antikörper

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• KD/KO Validated

KCNA3 Polyklonal Antikörper für WB, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14079-1-AP

Synonyme

Kv1.3, MK3, HUKIII, HPCN3, HLK3

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  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14079-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14079-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14079-1-AP (Kv1.3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of Rat brain using 14079-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Rat brain tissue using Kv1.3 antibody (14079-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Maus-Thymusgewebe
• Erfolgreiche Detektion in IF-P: Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 5 publications below

Produktinformation

14079-1-AP bindet in WB, IF-P, ELISA KCNA3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNA3 fusion protein Ag5204
• Vollständiger Name: potassium voltage-gated channel, shaker-related subfamily, member 3
• Berechnetes Molekulargewicht: 575 aa, 64 kDa
• Beobachtetes Molekulargewicht: 52 kDa, 75 kDa
• GenBank-Zugangsnummer: BC035059
• Gene symbol: KCNA3
• Gene ID (NCBI): 3738
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Kv1.3 ( also known as KCNA3, MK3, HGK5, HLK3, PCN3, HPCN3) contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T cell proliferation and activation. Kv1.3 channels are expressed in in various tissues and cell types including brain, vascular smooth muscle cells and leucocytes. Malfunction of Kv1.3 can alter the immune response, elicit neurotoxic effects or impact on cancer growth(PMID: 24305723; 24133455). 14079-1-AP can detect two isoforms of Kv1.3 other than the canonical channel (60 KDa) : A longer isoform (70 KDa) and a truncated isoform (43 KDa).

Publikationen

Species	Application	Title
mouse	WB	Cell Mol Immunol; NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.; Authors - Xiaolong He ; KD Validated
mouse	WB,IF	Int Immunopharmacol; Clarithromycin attenuates airway epithelial-mesenchymal transition in ovalbumin-induced asthmatic mice through modulation of Kv1.3 channels and PI3K/Akt signaling; Authors - Bingqing Sun
human	WB	FASEB J; The Kv1.3 ion channel acts as a host factor restricting viral entry.; Authors - Yange Lang
rat	WB	Int J Clin Exp Med; Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1Inhibition of 15-lipoxygenase (15-LOX) reverses hypoxia-induced down-regulation of potassium channels Kv1.5 and Kv2.1.; Authors - Wenjuan Liu
mouse	WB,IF	J Ethnopharmacol; Processed Buthus martensii Karsch scorpions ameliorate diet-induced NASH in mice by attenuating Kv1.3-mediated macrophage activation; Authors - Erjin Xu
human,mouse	WB,IF	Transl Res; Kv1.3 expression on CD4 (+) T cells promotes interleukin-17A-associated airway inflammation and airway remodeling in asthma; Authors - Bingqing Sun