KCNAB2 Polyklonaler Antikörper

KCNAB2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 17890-1-AP

Synonyme

AKR6A5, HKvbeta2, HKvbeta2.1, HKvbeta2.2, K(+) channel subunit beta 2, KCNA2B, KCNAB2, KCNK2, KV BETA 2, TREK, TREK1

---

• 

  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17890-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17890-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17890-1-AP (KCNAB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 17890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 17890-1-AP (KCNAB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe, Mausherzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

17890-1-AP bindet in WB, IHC, ELISA KCNAB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNAB2 fusion protein Ag12096
• Vollständiger Name: potassium voltage-gated channel, shaker-related subfamily, beta member 2
• Berechnetes Molekulargewicht: 395 aa, 44 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC110351
• Gene symbol: KCNAB2
• Gene ID (NCBI): 8514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	IHC	Sci Rep; The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states.; Authors - Guy Barry
human	WB	Brain Sci; Differential Expression of Multiple Disease-Related Protein Groups Induced by Valproic Acid in Human SH-SY5Y Neuroblastoma Cells.; Authors - Tsung-Ming Hu
human	WB	Cells; The Ion Channel Gene KCNAB2 Is Associated with Poor Prognosis and Loss of Immune Infiltration in Lung Adenocarcinoma; Authors - Yin Lyu
human	WB	Acta Biochim Biophys Sin (Shanghai); Screening of autoantibodies as biomarkers in the serum of renal cancer patients based on human proteome microarray; Authors - Yangyang Sun