Kv4.2 Rekombinanter Antikörper

Kv4.2 Rekombinant Antikörper für WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242754F4

Kat-Nr. : 85133-3-PBS

Synonyme

KCND2, 242754F4, KIAA1044, Potassium voltage-gated channel subfamily D member 2, RK5

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  WB analysis using 85133-3-RR (same clone as 85133-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  WB analysis of DU 145 using 85133-3-RR (same clone as 85133-3-PBS)

  DU 145 cells were subjected to SDS PAGE followed by western blot with 85133-3-RR (KCND2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 85133-3-RR (same clone as 85133-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 85133-3-RR (Kv4.2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 85133-3-RR (same clone as 85133-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using Kv4.2 antibody (85133-3-RR, Clone: 242754F4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85133-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01834-2

  Cytometric bead array standard curve of MP01834-2, Kv4.2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85133-4-PBS. Detection antibody: 85133-3-PBS. Standard: Ag15879. Range: 1.563-100 ng/mL.

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  Affinity and Kinetic Characterization of 85133-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85133-3-RR against Human Kv4.2 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

85133-3-PBS bindet in WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Kv4.2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Kv4.2 fusion protein Ag15879
• Vollständiger Name: potassium voltage-gated channel, Shal-related subfamily, member 2
• Berechnetes Molekulargewicht: 630 aa, 71 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC110449
• Gene symbol: KCND2
• Gene ID (NCBI): 3751
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Voltage-gated potassium or Kv channels, specifically those mediating low threshold, rapidly inactivating Ito and IA currents, are known to regulate cardiac and neuronal membrane excitability, respectively (PMID: 12829703). Voltage-gated potassium channel subunit Kv4.2, encoded by the KCND2 gene, belongs to the potassium channel family and D (Shal) subfamily. It is a pore-forming alpha subunit of voltage-gated rapidly inactivating A-type potassium channels. Kv4.2 is highly expressed in the brain (PMID: 10551270). It is a major constituent of A-type potassium currents and a key regulator of neuronal membrane excitability (PMID: 22539834).