KCND3 Polyklonaler Antikörper

KCND3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25468-1-AP

Synonyme

KV4.3, KSHIVB, KCND3S, KCND3L

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  WB analysis using 25468-1-AP

  Rat brain tissues (heated at 100 ° C, heated at 37℃) lysates were subjected to SDS PAGE followed by western blot with 25468-1-AP (KCND3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 25468-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25468-1-AP (KCND3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe, 37℃ incubated rat brain tissue
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maus-Cerebellum-Gewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

25468-1-AP bindet in WB, IHC, ELISA KCND3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCND3 fusion protein Ag22183
• Vollständiger Name: potassium voltage-gated channel, Shal-related subfamily, member 3
• Berechnetes Molekulargewicht: 655 aa, 73 kDa
• Beobachtetes Molekulargewicht: 73 kDa
• GenBank-Zugangsnummer: BC113477
• Gene symbol: KCND3
• Gene ID (NCBI): 3752
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCND3 gene encodes the voltage-gated potassium ion channel subfamily D member 3, a six trans-membrane protein (Kv4.3), involved in the transient outward K+ current. KCND3 gene defect causes both cardiological and neurological syndromes. Kv4.3 contributes to the cardiac transient outward potassium current (Ito1), the main contributing current to the repolarizing phase 1 of the cardiac action potential.