KCNH1 Polyklonaler Antikörper

KCNH1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26426-1-AP

Synonyme

EAG, EAG channel 1, EAG1, Ether-a-go-go potassium channel 1, h eag

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  WB analysis using 26426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26426-1-AP (KCNH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of rat brain using 26426-1-AP

  IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with rat brain tissue lysate 1720 ug.

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  IP experiment of mouse brain using 26426-1-AP

  IP result of anti-KCNH1 (IP:26426-1-AP, 4ug; Detection:26426-1-AP 1:5000) with mouse brain tissue lysate 2120 ug.

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  IHC staining of mouse brain using 26426-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 26426-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 26426-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26426-1-AP (KCNH1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26426-1-AP bindet in WB, IHC, ELISA KCNH1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNH1 fusion protein Ag16647
• Vollständiger Name: potassium voltage-gated channel, subfamily H (eag-related), member 1
• Berechnetes Molekulargewicht: 989 aa, 111 kDa
• Beobachtetes Molekulargewicht: 111 kDa
• GenBank-Zugangsnummer: BC113709
• Gene symbol: KCNH1
• Gene ID (NCBI): 3756
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).