KCNH7 Rekombinanter Antikörper

KCNH7 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240644B5

Kat-Nr. : 83674-1-RR

Synonyme

ERG-3, ERG3, ERG 3, Eag-related protein 3, Eag related protein 3

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  WB analysis using 83674-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 83674-1-RR

  pig brain tissue were subjected to SDS PAGE followed by western blot with 83674-1-RR (KCNH7 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 83674-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 83674-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 83674-1-RR (KCNH7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83674-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83674-1-RR against Human KCNH7 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Hausschwein-Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83674-1-RR bindet in WB, IHC, ELISA KCNH7 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KCNH7 fusion protein Ag4532
• Vollständiger Name: potassium voltage-gated channel, subfamily H (eag-related), member 7
• Berechnetes Molekulargewicht: 1196 aa, 135 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC035815
• Gene symbol: KCNH7
• Gene ID (NCBI): 90134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Potassium voltage-gated channel subfamily H member 7 is a protein that in humans is encoded by the KCNH7 gene. The protein encoded by this gene is a voltage-gated potassium channel subunit. KCNH7 is pore-forming (alpha) subunit of voltage-gated potassium channel. Channel properties may be modulated by cAMP and subunit assembly.