KCNIP3 Polyklonaler Antikörper

KCNIP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 12032-1-AP

Synonyme

A-type potassium channel modulatory protein 3, Calsenilin, DRE-antagonist modulator, Kv channel-interacting protein 3

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  WB analysis of mouse brain using 12032-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12032-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12032-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12032-1-AP

  IP result of anti-KCNIP3 (IP:12032-1-AP, 4ug; Detection:12032-1-AP 1:1000) with mouse brain tissue lysate 4400 ug.

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  IHC staining of human gliomas using 12032-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 12032-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of SH-SY5Y using 12032-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KCNIP3 antibody (12032-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Hirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12032-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA KCNIP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNIP3 fusion protein Ag2665
• Vollständiger Name: Kv channel interacting protein 3, calsenilin
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa, 27 kDa
• GenBank-Zugangsnummer: BC012850
• Gene symbol: KCNIP3
• Gene ID (NCBI): 30818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology.

Publikationen

Species	Application	Title
Human		Mol Med Rep; Protective effects of hydrogen sulfide against angiotensin II-induced endoplasmic reticulum stress in HUVECs.; Authors - Heng-Jing Hu