KCNIP3 Polyklonaler Antikörper

KCNIP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12032-1-PBS

Synonyme

A-type potassium channel modulatory protein 3, Calsenilin, DRE-antagonist modulator, Kv channel-interacting protein 3

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  WB analysis of mouse brain using 12032-1-AP (same clone as 12032-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 12032-1-AP (same clone as 12032-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 12032-1-AP (same clone as 12032-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12032-1-AP (KCNIP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12032-1-AP (same clone as 12032-1-PBS)

  IP result of anti-KCNIP3 (IP:12032-1-AP, 4ug; Detection:12032-1-AP 1:1000) with mouse brain tissue lysate 4400 ug. This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12032-1-AP (same clone as 12032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 12032-1-AP (same clone as 12032-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12032-1-AP (KCNIP3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 12032-1-AP (same clone as 12032-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using KCNIP3 antibody (12032-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12032-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12032-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA KCNIP3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNIP3 fusion protein Ag2665
• Vollständiger Name: Kv channel interacting protein 3, calsenilin
• Berechnetes Molekulargewicht: 256 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa, 27 kDa
• GenBank-Zugangsnummer: BC012850
• Gene symbol: KCNIP3
• Gene ID (NCBI): 30818
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNIP3 also known as Calsenilin, CSEN, DREAM, or KCHIP3, is a protein-coding gene located on human chromosome 2q11.1 . It encodes a small calcium-binding protein belonging to the recoverin branch of the EF-hand superfamily. KCNIP3 is an integral subunit of native Kv4 channel complexes, regulating A-type potassium currents in response to intracellular calcium changes, thereby modulating neuronal excitability. KCNIP3 interacts with presenilin 1 (PSEN1) and presenilin 2 (PSEN2), influencing γ-secretase activity and β-amyloid formation, potentially linking it to Alzheimer's disease pathology.