KCNIP4 Monoklonaler Antikörper

KCNIP4 Monoklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

5B1D12

Kat-Nr. : 60133-1-Ig

Synonyme

A-type potassium channel modulatory protein 4, CALP, Calsenilin like protein, Calsenilin-like protein, KCHIP4

---

• 

  WB analysis of pig brain using 60133-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 60133-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60133-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 60133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 60133-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60133-1-Ig (KCNIP4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 60133-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of mouse brain using 60133-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KCNIP4 antibody (60133-1-Ig, Clone: 5B1D12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, humanes Hirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 2 publications below

Produktinformation

60133-1-Ig bindet in WB, IHC, IF-P, ELISA KCNIP4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KCNIP4 fusion protein Ag4863
• Vollständiger Name: Kv channel interacting protein 4
• Berechnetes Molekulargewicht: 250 aa, 28 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC032520
• Gene symbol: KCNIP4
• Gene ID (NCBI): 80333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNIP4 is a member of the family of voltage-gated potassium (Kv) channel-interacting proteins (KCNIPs), which belong to the recoverin branch of the EF-hand superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium.

Publikationen

Species	Application	Title
mouse	IF	iScience; Single-nucleus RNA sequencing-based construction of a hippocampal neuron atlas in mice with epileptic cognitive impairment; Authors - Jia-Qi Ma
mouse,human	WB,IF	bioRxiv; Molecular Signatures of Resilience to Alzheimer's Disease in Neocortical Layer 4 Neurons; Authors - S Akila Parvathy Dharshini