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KCNJ1 Polyklonaler Antikörper

KCNJ1 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20953-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    WB analysis of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20953-1-AP (KCNJ1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using KCNJ1 Polyclonal antibody (20953-1-AP)

    IP experiment of mouse brain using 20953-1-AP (same clone as 20953-1-PBS)

    IP result of anti-KCNJ1 (IP:20953-1-AP, 4ug; Detection:20953-1-AP 1:300) with mouse brain tissue lysate 3600ug. This data was developed using the same antibody clone with 20953-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20953-1-PBS bindet in WB, IP, Indirect ELISA KCNJ1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen KCNJ1 fusion protein Ag15100
Vollständiger Name potassium inwardly-rectifying channel, subfamily J, member 1
Berechnetes Molekulargewicht 391 aa, 45 kDa
Beobachtetes Molekulargewicht 75-80 kDa
GenBank-ZugangsnummerBC074752
Gene symbol KCNJ1
Gene ID (NCBI) 3758
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNJ1, also called ROMK, is an inward-rectifying K+ channel responsible for basal (non-flow stimulated) K+ secretion. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium. This channel is activated by internal ATP and can be blocked by external barium. The calculated molecule weight is 45kDa, 20953-1-AP can detect the band around 75-80 kDa, it has been considered to be a dimer of KCNJ1.(PMID: 17804670; 7929082; 15767585)