Kir2.1 Polyklonaler Antikörper

Kir2.1 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 19965-1-AP

Synonyme

HHBIRK1, HHIRK1, hIRK1, IRK 1, IRK1, KCNJ2, Kir2.1, LQT7, SQT3

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Galerie der Validierungsdaten

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  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

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  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

19965-1-AP bindet in WB, IHC, ELISA Kir2.1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: potassium inwardly-rectifying channel, subfamily J, member 2
• Berechnetes Molekulargewicht: 48 kDa
• Beobachtetes Molekulargewicht: 50 kDa, 60 kDa
• GenBank-Zugangsnummer: NM_000891
• Gene symbol: KCNJ2
• Gene ID (NCBI): 3759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNJ2, also named as HHBIRK1, HHIRK1, IRK1, KIR2.1, LQT7 and SQT3, belongs to the inward rectifier-type potassium channel family. KCNJ2 probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ2 can be blocked by extracellular barium or cesium. Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7). Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3). The antibody recognizes the C-term of KCNJ2.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	WB	Stem Cell Res Ther; Overexpression of KCNJ2 enhances maturation of human-induced pluripotent stem cell-derived cardiomyocytes; Authors - Jingjun Zhou
rabbit	WB	J Mol Cell Cardiol; Altered long non-coding RNA expression profile in rabbit atria with atrial fibrillation: TCONS_00075467 modulates atrial electrical remodeling by sponging miR-328 to regulate CACNA1C.; Authors - Zhan Li
rabbit	WB	J Cell Mol Med; Long noncoding RNA TCONS-00106987 promotes atrial electrical remodelling during atrial fibrillation by sponging miR-26 to regulate KCNJ2.; Authors - Juanjuan Du
rat	WB	J Cell Mol Med; Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.; Authors - Yi Guan
human	WB,IHC	Cell Commun Signal; KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma; Authors - Mao Shen