KCNK18 Polyklonaler Antikörper

KCNK18 Polyklonal Antikörper für IF, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23712-1-AP

Synonyme

K2p18.1, KCNK18, TRESK, TRESK 2, TRESK2, TRIK

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Galerie der Validierungsdaten

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  WB analysis of A431 using 23712-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 23712-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23712-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 23712-1-AP (KCNK18 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 23712-1-AP

  Immunofluorescent analysis of A375 cells using 23712-1-AP (KCNK18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23712-1-AP bindet in WB, IF,ELISA KCNK18 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNK18 fusion protein Ag20514
• Vollständiger Name: potassium channel, subfamily K, member 18
• Berechnetes Molekulargewicht: 384 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC152979
• Gene symbol: KCNK18
• Gene ID (NCBI): 338567
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNK18, also named as TRESK and TRIK, is an outward rectifying potassium channel which produces rapidly activating outward rectifier K+ currents. It may function as background potassium channel that sets the resting membrane potential. KCNK18 is inhibited by arachidonic acid and other naturally occurring unsaturated free fatty acids. KCNK18 channel activity is enhanced by volatile anesthetics, such as isoflurane. The gene of KCNK18 maps to chromosome 10q26.11, and encodes a 384-amino acid protein with a predicted unmodified molecular weight of 44 kDa. This antibody can be used to detect endogenous KCNK18, however, the reason for the additional band of 130-140 kDa is still unknown.