KCNMA1 Polyklonaler Antikörper

KCNMA1 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22158-1-AP

Synonyme

BK channel, BKCA alpha, BKTM, hSlo, K(VCA)alpha

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  WB analysis using 22158-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 22158-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 22158-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mouse brain membrane protein, rat brain membrane protein
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, Maushirngewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

22158-1-AP bindet in WB, IHC, IF-P, ELISA KCNMA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNMA1 fusion protein Ag17802
• Vollständiger Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
• Berechnetes Molekulargewicht: 1236 aa, 138 kDa
• Beobachtetes Molekulargewicht: ~130 kDa
• GenBank-Zugangsnummer: BC137115
• Gene symbol: KCNMA1
• Gene ID (NCBI): 3778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.