KCNMA1 Polyklonaler Antikörper

KCNMA1 Polyklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22158-1-PBS

Synonyme

BK channel, BKCA alpha, BKTM, hSlo, K(VCA)alpha

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  WB analysis using 22158-1-AP (same clone as 22158-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 22158-1-AP (KCNMA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 22158-1-AP (same clone as 22158-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22158-1-AP (KCNMA1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 22158-1-AP (same clone as 22158-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KCNMA1 antibody (22158-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22158-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22158-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA KCNMA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNMA1 fusion protein Ag17802
• Vollständiger Name: potassium large conductance calcium-activated channel, subfamily M, alpha member 1
• Berechnetes Molekulargewicht: 1236 aa, 138 kDa
• Beobachtetes Molekulargewicht: ~130 kDa
• GenBank-Zugangsnummer: BC137115
• Gene symbol: KCNMA1
• Gene ID (NCBI): 3778
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated potassium channel subunit alpha-1 also known as large conductance calcium-activated potassium channel, subfamily M, alpha member 1 (KCa1.1), or BK channel alpha subunit, is a voltage-gated potassium channel encoded by the KCNMA1 gene and characterized by their large conductance of potassium ions (K+) through cell membranes. KCNMA1 is widely distributed in human cell membranes, especially in muscle cells and neurons. They regulate the excitability of cells through sensitivity to calcium ions (Ca²⁺) and voltage, thereby affecting key physiological processes such as muscle contraction and nerve signal transduction.