KCNMB1 Polyklonaler Antikörper

KCNMB1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12933-1-AP

Synonyme

Calcium-activated potassium channel subunit beta-1, Calcium-activated potassium channel subunit beta, BKbeta1, BKbeta, BK channel subunit beta-1

---

• 

  WB analysis using 12933-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12933-1-AP (KCNMB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maus-Skelettmuskelgewebe, Rattenhirngewebe, Ratten-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

12933-1-AP bindet in WB, ELISA KCNMB1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNMB1 fusion protein Ag3888
• Vollständiger Name: potassium large conductance calcium-activated channel, subfamily M, beta member 1
• Berechnetes Molekulargewicht: 130aa,15 kDa; 190aa,22 kDa
• Beobachtetes Molekulargewicht: 31 kDa
• GenBank-Zugangsnummer: BC025707
• Gene symbol: KCNMB1
• Gene ID (NCBI): 3779
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Calcium-activated potassium channel subunit beta-1 is a protein encoded by KCNMB1 gene, also named as BK beta1, slo beta1. MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Beta subunits (beta 1-4) are highly tissue specific in their expression, with beta-1 being present predominantly on vascular smooth muscle. Endothelial cells are not known to express beta1, subunits. Beta1, is also known to be expressed in urinary bladder and in some regions of the brain. Association of the beta-1 subunit with the BK channel increases the apparent Ca2+ sensitivity of the channel and decreases voltage dependence.PMID 25015960