KCNMB4 Monoklonaler Antikörper

KCNMB4 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

4H7G3

Kat-Nr. : 60122-1-Ig

Synonyme

4H7G3, BK channel subunit beta 4, BK channel subunit beta-4, BKbeta4, Calcium-activated potassium channel subunit beta-4

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  WB analysis of fetal human brain using 60122-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 60122-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60122-1-Ig (KCNMB4 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60122-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60122-1-Ig (KCNMB4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: fetales humanes Hirngewebe, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

60122-1-Ig bindet in WB, IHC, ELISA KCNMB4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KCNMB4 fusion protein Ag5618
• Vollständiger Name: potassium large conductance calcium-activated channel, subfamily M, beta member 4
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC042446
• Gene symbol: KCNMB4
• Gene ID (NCBI): 27345
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Biomed Pharmacother; Cytoprotective effects of the flavonoid quercetin by activating mitochondrial BKCa channels in endothelial cells.; Authors - Rafał Paweł Kampa
human	WB	Mitochondrion; Deficiency of the BKCa potassium channel displayed significant implications for the physiology of the human bronchial epithelium; Authors - Kamila Maliszewska-Olejniczak
		Peptides; Apelin/APJ increased renal blood flow through endothelial BKCa channel induced p-eNOS and ET-1 in diabetic conditions; Authors - Mingcong Huang