KCNN4 Polyklonaler Antikörper

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• KD/KO Validated

KCNN4 Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 23271-1-AP

Synonyme

KCa3.1, KCNN4, SK4

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 23271-1-AP

  WB result of KCNN4 antibody (23271-1-AP, 1:4000) with si-control and si-KCNN4 transfected HEK293 cells.

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  WB analysis using 23271-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 23271-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 23271-1-AP (KCNN4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using KCNN4 antibody (23271-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HEK-293 using 23271-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 23271-1-AP (KCNN4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• FC: See 1 publications below

Produktinformation

23271-1-AP bindet in WB, IHC, IF, FC, ELISA KCNN4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCNN4 fusion protein Ag19774
• Vollständiger Name: potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4
• Berechnetes Molekulargewicht: 427 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC015337
• Gene symbol: KCNN4
• Gene ID (NCBI): 3783
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The intermediate conductance calcium-activated potassium channel KCNN4, also known as KCa3.1, IK1, or SK4, is a member of the potassium channel KCNN family. KCNN4 is expressed in T cells, mast cells, macrophages, erythrocytes, vascular smooth muscle cells, airway smooth muscle cells, intestinal and airway epithelial cells (PMID: 19681865; 21493782). Functionally, KCNN4 plays a key role in calcium-dependent cell functions, such as proliferation, activation, and migration, in a broad range of cell types (PMID: 21493782).

Publikationen

Species	Application	Title
human	IF	Cancer Cell Int; KCNN4 may weaken anti-tumor immune response via raising Tregs and diminishing resting mast cells in clear cell renal cell carcinoma.; Authors - Yankang Cui
human	WB,IHC,FC	Cancer Biomark; KCNN4 promotes the progression of lung adenocarcinoma by activating the AKT and ERK signaling pathways; Authors - Ping Xu ; KD Validated
human	WB	Front Physiol; New KCNN4 Variants Associated With Anemia: Stomatocytosis Without Erythrocyte Dehydration; Authors - B. Allegrini
human	IHC	Biochem Biophys Res Commun; Integrated expression profiling of potassium channels identifys KCNN4 as a prognostic biomarker of pancreatic cancer.; Authors - Shuheng Jiang
mouse	WB	Gels; Comprehensive Optimization of Western Blotting; Authors - Dishiwen Liu
human	WB	Int J Mol Sci; Downregulation of IL-8 and IL-10 by the Activation of Ca2+-Activated K+ Channel KCa3.1 in THP-1-Derived M2 Macrophages; Authors - Susumu Ohya