KCNQ3 Polyklonaler Antikörper

KCNQ3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 19966-1-AP

Synonyme

Potassium voltage-gated channel subfamily KQT member 3, KV7.3, KQT-like 3, KQT like 3, EBN2

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  WB analysis of pig heart using 19966-1-AP

  pig heart tissue were subjected to SDS PAGE followed by western blot with 19966-1-AP (KCNQ3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 19966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19966-1-AP (KCNQ3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19966-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19966-1-AP (KCNQ3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Herzgewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

19966-1-AP bindet in WB, IHC, ELISA KCNQ3 und zeigt Reaktivität mit Hausschwein, human, Maus

• Getestete Reaktivität: Hausschwein, human, Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: potassium voltage-gated channel, KQT-like subfamily, member 3
• Berechnetes Molekulargewicht: 97 kDa
• Beobachtetes Molekulargewicht: 90 kDa
• GenBank-Zugangsnummer: NM_004519
• Gene symbol: KCNQ3
• Gene ID (NCBI): 3786
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCNQ3, also named as BFNC2, EBN2 and KV7.3, belongs to the potassium channel family and KQT subfamily. KCNQ3 is probably important in the regulation of neuronal excitability. Associates with KCNQ2 or KCNQ5, KCNQ3 forms a potassium channel with essentially identical properties to the channel underlying the native M-current, a slowly activating and deactivating potassium conductance which plays a critical role in determining the subthreshold electrical excitability of neurons as well as the responsiveness to synaptic inputs. Defects in KCNQ3 are the cause of benign neonatal epilepsy type 2 (EBN2). The antibody recognizes the C-term of KCNQ3.

Publikationen

Species	Application	Title
mouse	WB	Hippocampus; Impacted spike frequency adaptation associated with reduction of KCNQ2/3 exacerbates seizure activity in temporal lobe epilepsy; Authors - Shicheng Jiang
	WB	Genes (Basel); Differential Dorsolateral Prefrontal Cortex Proteomic Profiles of Suicide Victims with Mood Disorders.; Authors - Alejandra Cabello-Arreola