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  • KD/KO Validated

KCTD10 Polyklonaler Antikörper

KCTD10 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 27279-1-AP

Synonyme

BTB/POZ domain-containing adapter for CUL3-mediated RhoA degradation protein 3, BTB/POZ domain-containing protein KCTD10, hBACURD3, MSTP028, Potassium channel tetramerization domain-containing protein 10



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, HeLa-Zellen, HuH-7-Zellen, Jurkat-Zellen, K-562-Zellen, MCF-7-Zellen, U2OS-Zellen
Erfolgreiche IPHuH-7-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, humanes Prostatahyperplasie-Gewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:4000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

KD/KOSee 3 publications below
WBSee 4 publications below

Produktinformation

27279-1-AP bindet in WB, IHC, IP, ELISA KCTD10 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen KCTD10 fusion protein Ag25971
Vollständiger Name potassium channel tetramerisation domain containing 10
Berechnetes Molekulargewicht 313 aa, 35 kDa
Beobachtetes Molekulargewicht 35 kDa
GenBank-ZugangsnummerBC040062
Gene symbol KCTD10
Gene ID (NCBI) 83892
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCTD10 is a member of the polymerase delta-interacting protein 1 (PDIP1) gene family, which consists of 3 members, PDIP1, KCTD10 and TNFAIP1. KCTD10 is inducible by TNF-α, interacts with PCNA and the small subunit (p50) of DNA polymerase δ. Previous reports have shown that KCTD10 is essential for mammalian development. Further functional studies showed that KCTD10 interacts with proliferating cell nuclear antigen (PCNA) and plays an important role in DNA synthesis.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for KCTD10 antibody 27279-1-APProtokoll herunterladen
IHC protocol for KCTD10 antibody 27279-1-APProtokoll herunterladenl
IP protocol for KCTD10 antibody 27279-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cell

Unbiased interrogation of functional lysine residues in human proteome

Authors - Ying Bao
humanWB

Nat Commun

Regulation of TRIF-mediated innate immune response by K27-linked polyubiquitination and deubiquitination.

Authors - Xin Wu
  • KO Validated
mouseWB

Front Cell Dev Biol

Cullin3-TNFAIP1 E3 Ligase Controls Inflammatory Response in Hepatocellular Carcinoma Cells via Ubiquitination of RhoB.

Authors - Yue Liu
  • KD Validated
rat,humanWB

Genet Res (Camb)

Inhibition of KCTD10 Affects Diabetic Retinopathy Progression by Reducing VEGF and Affecting Angiogenesis

Authors - Yun Feng
  • KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Vasu (Verified Customer) (09-12-2025)

This antibody is suitable for few applications like immunofluroscence and Immunoblotting but not for other applications like Chip and flow cytometry and other applications

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry, Protein purification
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Breast and Cervical Cancer cells
FH

Vasudevarao (Verified Customer) (09-04-2025)

This antibody works very well for immunoblotting and IHC at the recommended dilutions

  • Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate and Breast Cancer cells