KCTD12 Polyklonaler Antikörper

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KCTD12 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15523-1-PBS

Synonyme

BTB/POZ domain-containing protein KCTD12, C13orf2, PFET1, Pfetin

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  WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15523-1-AP (same clone as 15523-1-PBS)

  Hela cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  WB analysis of rat brain using 15523-1-AP (same clone as 15523-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 15523-1-AP (same clone as 15523-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 15523-1-AP (same clone as 15523-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 15523-1-AP (same clone as 15523-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15523-1-AP (KCTD12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human bowen disease using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IHC staining of human intrahepatic cholangiocarcinoma using 15523-1-AP (same clone as 15523-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 15523-1-AP (KCTD12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 15523-1-AP (same clone as 15523-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 15523-1-AP (same clone as 15523-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KCTD12 antibody (15523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15523-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15523-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA KCTD12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCTD12 fusion protein Ag5716
• Vollständiger Name: potassium channel tetramerisation domain containing 12
• Berechnetes Molekulargewicht: 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC013764
• Gene symbol: KCTD12
• Gene ID (NCBI): 115207
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCTD12 also known as PFET1 or PFETIN, which contains a voltage-gated potassium (K+) channel tetramerization T1 domain and a BTB/POZ (Bric-a-brac, Tram-track, Broad complex poxvirus and zinc finger) domain, belongs to the KCTD family and was initially identified in cochlea. In addition to being a K+ channel protein that responds to membrane potential7, KCTD12 also acts as an auxiliary subunit of GABAB (γ-aminobutyric acid type B) receptors, which regulate emotionality and neuronal excitability.