KCTD14 Polyklonaler Antikörper

KCTD14 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21111-1-AP

Synonyme

BTB/POZ domain-containing protein KCTD14

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  WB analysis of mouse skeletal muscle using 21111-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 21111-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21111-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21111-1-AP (KCTD14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21111-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21111-1-AP (KCTD14 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, Maushirngewebe, Maus-Dünndarmgewebe
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

21111-1-AP bindet in WB, IHC, ELISA KCTD14 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCTD14 fusion protein Ag14265
• Vollständiger Name: potassium channel tetramerisation domain containing 14
• Berechnetes Molekulargewicht: 225 aa, 26 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001929
• Gene symbol: KCTD14
• Gene ID (NCBI): 65987
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCTD14, or potassium channel tetramerization domain containing 14, is a member of the KCTD family of proteins. This family consists of 25 members in humans, many of which are only partially characterized . KCTD14, like other members of the KCTD family, contains a conserved domain known as the BTB (Broad complex, Tramtrak, and Bric-a-brac)/POZ (poxvirus zinc finger) domain, which is crucial for protein oligomerization and establishing protein-protein interactions. KCTD14 has been implicated in various biological functions, and recent research suggests a potential role in cancer. Although not as extensively studied as some other KCTD members, KCTD14 has been noted in database analyses to potentially play a protumor role in ovarian cancer. Specifically, the COSMIC database reports a copy number variation (CNV) gain in 4.5% of ovarian cancers, and the GENT2 database indicates a fold change (FC) of 1.5 in expression, with a p-value less than 0.001, suggesting a possible association with ovarian cancer.