KCTD15 Polyklonaler Antikörper

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• KD/KO Validated

KCTD15 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20128-1-AP

Synonyme

BTB/POZ domain-containing protein KCTD15, Potassium channel tetramerization domain-containing protein 15

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  WB analysis using 20128-1-AP

  Mouse brain tissues were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20128-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 20128-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 20128-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 20128-1-AP (KCTD15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 20128-1-AP

  IP result of anti-KCTD15 (IP:20128-1-AP, 4ug; Detection:20128-1-AP 1:500) with C6 cells lysate 1960 ug.

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  IHC staining of mouse spleen using 20128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 20128-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 20128-1-AP (KCTD15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, C6-Zellen, HEK-293-Zellen, Mauslungengewebe
• Erfolgreiche IP: C6-Zellen
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

20128-1-AP bindet in WB, IHC, IP, ELISA KCTD15 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KCTD15 fusion protein Ag13962
• Vollständiger Name: potassium channel tetramerisation domain containing 15
• Berechnetes Molekulargewicht: 283 aa, 32 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC001185
• Gene symbol: KCTD15
• Gene ID (NCBI): 79047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KCTD15 is a member of the KCTD family of proteins, which are involved in various biological processes. KCTD15 plays a role in embryonic development by regulating the neural crest domain. It inhibits neural crest induction by repressing the Wnt/β-catenin signaling pathway. KCTD15 is involved in the NF-κB signaling pathway, which is crucial for immune responses and inflammation. Its deregulation can influence the activation of the IKK-β enzyme complex, triggering the NF-κB pathway. KCTD15's expression levels in peripheral blood cells and leukemia cells suggest its potential as a biomarker for diagnosing and monitoring acute leukemias. Its distinct expression profile in different types of white blood cells highlights its importance in both physiological and pathological processes.

Publikationen

Species	Application	Title
human	WB	Oncogenesis; KCTD15 inhibits the Hedgehog pathway in Medulloblastoma cells by increasing protein levels of the oncosuppressor KCASH2.; Authors - Eleonora Spiombi ; KD Validated
		Proc Natl Acad Sci U S A; KCTD1 regulation of Adenylyl cyclase type 5 adjusts striatal cAMP signaling; Authors - Yini Liao