KChIP1 Monoklonaler Antikörper

KChIP1 Monoklonal Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3D6C1

Kat-Nr. : 66439-1-PBS

Synonyme

KCHIP1, KCNIP1, VABP, Vesicle APC binding protein

---

• 

  WB analysis of human cerebellum using 66439-1-Ig (same clone as 66439-1-PBS)

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 66439-1-Ig (same clone as 66439-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66439-1-Ig (KChIP1 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66439-1-Ig (KChIP1 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 66439-1-Ig (same clone as 66439-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using KChIP1 antibody (66439-1-Ig, Clone: 3D6C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66439-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

66439-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA KChIP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KChIP1 fusion protein Ag5494
• Vollständiger Name: Kv channel interacting protein 1
• Berechnetes Molekulargewicht: 227 aa, 27 kDa
• Beobachtetes Molekulargewicht: 25 kDa
• GenBank-Zugangsnummer: BC050375
• Gene symbol: KCNIP1
• Gene ID (NCBI): 30820
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human K(v) channel interacting protein 1 (KCHIP1) is a new member of the neural calcium binding protein superfamily. Members of the KCNIP family are small calcium binding proteins. They all have EF-hand-like domains, and differ from each other in the N-terminus. They are integral subunit components of native Kv4 channel complexes. They may regulate A-type currents, and hence neuronal excitability, in response to changes in intracellular calcium. KChIP1 is a neuronal calcium sensor protein that is predominantly expressed at GABAergic synapses and it has been related with modulation of K(+) channels, GABAergic transmission and cell death.