KDELC2 Polyklonaler Antikörper

KDELC2 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23345-1-AP

Synonyme

Protein O-xylosyltransferase POGLUT3, Protein O-glucosyltransferase 3, POGLUT3, KDEL motif-containing protein 2, EC:2.4.2.-

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  WB analysis of HeLa using 23345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23345-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 23345-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 23345-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 23345-1-AP (KDELC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 23345-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 23345-1-AP (KDELC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 23345-1-AP

  Immunofluorescent analysis of HepG2 cells using 23345-1-AP (KDELC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Plazenta-Gewebe, SMMC-7721-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23345-1-AP bindet in WB, IHC, IF/ICC, ELISA KDELC2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KDELC2 fusion protein Ag19929
• Vollständiger Name: KDEL (Lys-Asp-Glu-Leu) containing 2
• Berechnetes Molekulargewicht: 507 aa, 59 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC036526
• Gene symbol: KDELC2
• Gene ID (NCBI): 143888
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.