KDM1 Monoklonaler Antikörper

KDM1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2F10A12

Kat-Nr. : 67037-1-PBS

Synonyme

AOF2, BHC110, KDM1, KDM1A, KIAA0601, LSD1, lysine (K) demethylase 1

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  WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67037-1-Ig (same clone as 67037-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67037-1-Ig (KDM1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 67037-1-Ig (same clone as 67037-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67037-1-Ig (KDM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67037-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67037-1-PBS bindet in WB, IHC, Indirect ELISA KDM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KDM1 fusion protein Ag14778
• Vollständiger Name: lysine (K)-specific demethylase 1
• Berechnetes Molekulargewicht: 876 aa, 95 kDa
• Beobachtetes Molekulargewicht: 110 kDa
• GenBank-Zugangsnummer: BC040194
• Gene symbol: KDM1
• Gene ID (NCBI): 23028
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Lysine specific demethylase 1 (LSD1/BHC110/KIAA0601/p110b/AOF2/KDM1) is an amine oxidase that catalyzes histone demethylation via a flavin adenine dinucleotide (FAD)-dependent oxidative reaction(PMID:19703393). This protein belongs to the flavin monoamine oxidase family. Its structure and function is conserved from yeast to human and it is typically associated to CoREST, a corepressor protein, and histone deacetylases HDAC1 and HDAC2. Human LSD1 consists of 852 amino acids and comprises an N-terminal SWIRM domain, involved in protein interactions, and a C-terminal amine oxidase domain, which contains an insertion that forms the CoREST interacting site (the so-called tower domain )(PMID:20164337). It has 2 isoforms produced by alternative splicing with the calculated molecular mass of 93-95 kDa and apparent molecular mass of 110 kDa.