KDM5C Polyklonaler Antikörper

KDM5C Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 14426-1-AP

Synonyme

Lysine-specific demethylase 5C, Jumonji/ARID domain-containing protein 1C, JARID1C, EC:1.14.11.67, DXS1272E

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  WB analysis using 14426-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14426-1-AP (KDM5C antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 14426-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14426-1-AP (KDM5C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MCF-7-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

14426-1-AP bindet in WB, IF/ICC, IP, ELISA KDM5C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KDM5C fusion protein Ag5791
• Vollständiger Name: lysine (K)-specific demethylase 5C
• Berechnetes Molekulargewicht: 176 kDa
• Beobachtetes Molekulargewicht: 171-180 kDa
• GenBank-Zugangsnummer: BC054499
• Gene symbol: KDM5C
• Gene ID (NCBI): 8242
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).

Publikationen

Species	Application	Title
human	WB	Sci Adv; EIF3D safeguards the homeostasis of key signaling pathways in human primed pluripotency; Authors - Chikako Okubo
human	WB,IP	Cancer Res; KDM5C-mediated recruitment of BRD4 to chromatin regulates enhancer activation and BET inhibitor sensitivity; Authors - Yulong Qiang
human	WB	Cell Death Dis; USP10 stabilizes BAZ1A to drive tumor stemness via an epigenetic mechanism in head and neck squamous cell carcinoma; Authors - Yanni Shi
human	WB	Int J Biol Sci; KDM5B demethylates H3K4 to recruit XRCC1 and promote chemoresistance.; Authors - Wenxia Xu
human	WB,IF	Neurol Genet; Novel Variations in the KDM5C Gene Causing X-Linked Intellectual Disability.; Authors - Po-Ming Wu
mouse	WB	Immunohorizons; Inhibition of H3K27me3 Demethylases Promotes Plasmablast Formation.; Authors - Anna K Kania