KDM5C Rekombinanter Antikörper

KDM5C Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250484C10

Kat-Nr. : 86017-1-RR

Synonyme

[histone H3]-trimethyl-L-lysine(4) demethylase 5C, DXS1272E, EC:1.14.11.67, JARID1C, Jumonji/ARID domain-containing protein 1C

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  WB analysis using 86017-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86017-1-RR (KDM5C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86017-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86017-1-RR against Human KDM5C were performed. The affinity constant is 0.148 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86017-1-RR bindet in WB, ELISA KDM5C und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KDM5C fusion protein Ag5791
• Vollständiger Name: lysine (K)-specific demethylase 5C
• Berechnetes Molekulargewicht: 176 kDa
• Beobachtetes Molekulargewicht: 171-180 kDa
• GenBank-Zugangsnummer: BC054499
• Gene symbol: KDM5C
• Gene ID (NCBI): 8242
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDM5C belongs to the ARID protein family that harbors conserved DNA-binding motifs and acts as a histone H3 lysine 4 demethylase, suggesting a potential role in epigenetic regulation during development, cell differentiation (PMID:21575681). KDM5C protein is homologous to three other proteins of the human JARID1 family (JARID1A, JARID1B and JARID1C). This family shares several evolutionarily conserved domains, including the catalytic JmjC domain, JmjN domain, Arid/Bright domain, C5HC2 zinc-finger domain and two plant homeodomains (PHD), a motif that binds methyl-lysine residues (PMID:17320160,17320161).