VEGFR2/CD309 Rekombinanter Antikörper

VEGFR2/CD309 Rekombinant Antikörper für WB, IHC, FC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, FC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230238D7

Kat-Nr. : 83049-4-PBS

Synonyme

KDR, VEGFR2, VEGFR2/KDR, 230238D7, CD309

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  WB analysis of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  human placenta tissue was subjected to SDS PAGE followed by western blot with 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

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  IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human rectal cancer using 83049-4-RR (same clone as 83049-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human rectal cancer tissue slide using 83049-4-RR (VEGFR2/CD309 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

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  FC experiment of HUVEC using 83049-4-RR (same clone as 83049-4-PBS)

  1x10^6 HUVEC cells were surface stained with 0.25 ug VEGFR2/CD309 Recombinant antibody (83049-4-RR, Clone:230238D7) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were not fixed. This data was developed using the same antibody clone with 83049-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83049-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83049-4-RR against Human KDR were performed. The affinity constant is 4.71 nM.

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Geprüfte Anwendungen

Produktinformation

83049-4-PBS bindet in WB, IHC, FC, Indirect ELISA VEGFR2/CD309 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: VEGFR2/CD309 fusion protein Eg0218
• Vollständiger Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Berechnetes Molekulargewicht: 152 kDa
• Beobachtetes Molekulargewicht: 150 kDa, 230 kDa
• GenBank-Zugangsnummer: NM_002253
• Gene symbol: KDR
• Gene ID (NCBI): 3791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KDR, also named as VEGFR-2, FLK1 and CD309, is a receptor for VEGF or VEGFC. KDR which belongs to the protein kinase superfamily, has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. KDR functions as the main mediator of VEGF-induced endothelial proliferation, survival, migration, tubular morphogenesis and sprouting. Mutations of this gene are implicated in infantile capillary hemangiomas.