VEGFR2/KDR Rekombinanter Antikörper

VEGFR2/KDR Rekombinant Antikörper für Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

230238E8

Kat-Nr. : 83049-5-PBS

Synonyme

KDR, VEGFR2, Protein-tyrosine kinase receptor flk-1, FLK-1, EC:2.7.10.1

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  Sandwich ELISA standard curve of MP00012-3

  Sandwich ELISA standard curve of MP00012-3, Human VEGFR2/KDR Recombinant Matched Antibody Pair - PBS only. 83049-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0218.83049-5-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

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  Sample test of MP00012-3

  HUVEC was cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernatant was removed, assayed for human VEGFR2/KDR, and measured 267.3 pg/mL.

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  Sample test of MP00012-3

  The mean human VEGFR2/KDR concentration was determined to be 55,216.6 pg/mL in HUVEC cell extract based on a 0.4 mg/mL extract load.

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  Sample test of MP00012-3

  Plasma of eight individual healthy human donors was measured. The human VEGFR2/KDR concentration of detected samples was determined to be 5,643.5 ng/mL with a range of 1,988.2 - 9,946.0 pg/mL.

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Geprüfte Anwendungen

Produktinformation

83049-5-PBS bindet in Sandwich ELISA, Indirect ELISA, Sample test VEGFR2/KDR und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Fusionsprotein
• Vollständiger Name: kinase insert domain receptor (a type III receptor tyrosine kinase)
• Berechnetes Molekulargewicht: 152 kDa
• GenBank-Zugangsnummer: NM_002253
• Gene symbol: KDR
• Gene ID (NCBI): 3791
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.