KERA Rekombinanter Antikörper

KERA Rekombinant Antikörper für IF-P, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF-P, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

230399B11

Kat-Nr. : 83272-1-RR

Synonyme

Keratocan, 230399B11, CNA2, KTN, SLRR2B

---

• 

  IF Staining of rat eye using 83272-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using KERA antibody (83272-1-RR, Clone: 230399B11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of U251 using 83272-1-RR

  1x10^6 U251 were intracellularly stained with 0.2 ug KERA Recombinant Antibody (83272-1-RR, Clone:230399B11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83272-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83272-1-RR against Human KERA were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: Ratten-Augengewebe
• Erfolgreiche Detektion in FC (Intra): U251

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83272-1-RR bindet in IF-P, FC (Intra), ELISA KERA und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KERA fusion protein Ag34171
• Vollständiger Name: keratocan
• Berechnetes Molekulargewicht: 352 aa, 41 kDa
• GenBank-Zugangsnummer: BC032667
• Gene symbol: KERA
• Gene ID (NCBI): 11081
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Keratocan and Lumican are the predominant class II SLRPs(Small leucine-rich proteoglycans) in the corneal stroma. While not totally corneal-specific together these SLRPs are excellent biomarkers for the tissue. Mutant mice deficient for keratocan expression have transparent but thin corneal stromas with a mild increase in collagen fibril diameter with no further abnormalities.In humans, mutations in the keratocan gene are associated with a flattened cornea in a rare condition known as cornea plana. The relative specificity of keratocan to corneal keratocytes has been useful for tissue-specific targeting for genetic manipulation of the corneal stroma.(PMID: 32663498)