KGA/GAC Monoklonaler Antikörper

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KGA/GAC Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A12A1

Kat-Nr. : 66265-1-PBS

Synonyme

GLS, KGA, 3A12A1, AAD20, EC:3.5.1.2

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  WB analysis of NIH/3T3 using 66265-1-Ig (same clone as 66265-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of rat liver using 66265-1-Ig (same clone as 66265-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 66265-1-Ig (same clone as 66265-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of U-251 using 66265-1-Ig (same clone as 66265-1-PBS)

  U-251 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66265-1-Ig (same clone as 66265-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66265-1-Ig (GLS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66265-1-Ig (GLS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66265-1-Ig (GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KGA/GAC antibody (66265-1-Ig, Clone: 3A12A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66265-1-Ig (same clone as 66265-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HepG2 cells using 66265-1-Ig(GLS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66265-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66265-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA KGA/GAC und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KGA/GAC fusion protein Ag24227
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 669 aa, 73 kDa
• Beobachtetes Molekulargewicht: 58 kDa, 65 kDa
• GenBank-Zugangsnummer: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease(PMID:1672808).The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID:12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). It has 3 isoforms named as KGA, GAM, GAC. This antibody can recognize KGA and GAC.