KGA-Specific Monoklonaler Antikörper

KGA-Specific Monoklonal Antikörper für IF, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B11A11

Kat-Nr. : CL488-66265

Synonyme

AAD20, DKFZp686O15119, FLJ10358, GAC, GLS, GLS1, glutaminase, K glutaminase, KGA-Specific, KIAA0838, L glutamine amidohydrolase

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Galerie der Validierungsdaten

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  WB analysis using CL488-66265

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-66265 (KGA-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

• 

  IF Staining of mouse brain using CL488-66265

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 KGA-Specific antibody (CL488-66265, Clone: 1B11A11 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, Maushirngewebe
• Erfolgreiche Detektion in IF: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-66265 bindet in WB, IF KGA-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: KGA-Specific fusion protein Ag24227
• Vollständiger Name: glutaminase
• Berechnetes Molekulargewicht: 669 aa, 73 kDa
• Beobachtetes Molekulargewicht: 65 kDa
• GenBank-Zugangsnummer: BC038507
• Gene symbol: GLS
• Gene ID (NCBI): 2744
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa. The bands for both cell lines are clearly different in size from both rat liver glutaminase (58 kDa) and rat kidney glutaminase (65 kDa)(PMID: 12408749). It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID: 447624;7512428). It has 3 isoforms produced by alternative splicing named as KGA, GAM, GAC. This antibody is specific to KGA.