KHDC1 Polyklonaler Antikörper

KHDC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23443-1-AP

Synonyme

NDG1, KHDC 1, KH homology domain-containing protein 1, C6orf148, C6orf147

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  WB analysis of L02 using 23443-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 23443-1-AP (KHDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 23443-1-AP (KHDC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach using 23443-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach slide using 23443-1-AP (KHDC1 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Magenkrebsgewebe, humanes Lebergewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

23443-1-AP bindet in WB, IHC, ELISA KHDC1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KHDC1 fusion protein Ag20115
• Vollständiger Name: KH homology domain containing 1
• Berechnetes Molekulargewicht: 237 aa, 27 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC022080
• Gene symbol: KHDC1
• Gene ID (NCBI): 80759
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHDC1, also named as C6orf148, belongs to the KHDC1 family. KHDC1 has two isoforms with calculated MW 27 kDa and 18 kDa. It's a membrane protein. We got 35-40 kDa in our WB detection.