KHDRBS3 Polyklonaler Antikörper

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• KD/KO Validated

KHDRBS3 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13563-1-AP

Synonyme

Etle, etoile, KHDRBS3, RNA binding protein T Star, SALP, Sam68 like mammalian protein 2, SLM 2, SLM2, T STAR, TSTAR

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 13563-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13563-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 13563-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 13563-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13563-1-AP (KHDRBS3 Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human gliomas using 13563-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 13563-1-AP (KHDRBS3 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, BGC-823-Zellen, humanes Hirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

13563-1-AP bindet in WB, IHC,ELISA KHDRBS3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KHDRBS3 fusion protein Ag4438
• Vollständiger Name: KH domain containing, RNA binding, signal transduction associated 3
• Berechnetes Molekulargewicht: 346 aa, 38, 8 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC032606
• Gene symbol: KHDRBS3
• Gene ID (NCBI): 10656
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3, SLM2) is a RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion (15901763). May play a role as a negative regulator of cell growth. Inhibits cell proliferation. Involved in splice site selection of vascular endothelial growth factor. Induces an increased concentration-dependent incorporation of exon in CD44 pre-mRNA by direct binding to purine-rich exonic enhancer. RNA-binding abilities are down-regulated by tyrosine kinase PTK6. Involved in post-transcriptional regulation of HIV-1 gene expression (11741900).Ubiquitous with higher expression in testis, skeletal muscle and brain. Expressed in the kidney only in podocytes, the glomerular epithelial cells of the kidney. Strongly expressed after meiosis.This antibody is a rabbit polyclonal antibody raised against a full-length human KHDRBS3 protein.The observed molecular weight of KHDRBS is about 55kd.

Publikationen

Species	Application	Title
human	IHC	Acta Neuropathol; Transportin 1 accumulates specifically with FET proteins but no other transportin cargos in FTLD-FUS and is absent in FUS inclusions in ALS with FUS mutations.; Authors - Neumann Manuela M
mouse	WB	PLoS Genet; The tissue-specific RNA binding protein T-STAR controls regional splicing patterns of neurexin pre-mRNAs in the brain.; Authors - Ehrmann Ingrid I
human	WB	Cell Death Dis; KHDRBS3 regulates the permeability of blood-tumor barrier via cDENND4C/miR-577 axis.; Authors - Peiqi Wu ; KD Validated