KHSRP Polyklonaler Antikörper

KHSRP Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31044-1-AP

Synonyme

Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2, KH type-splicing regulatory protein

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  WB analysis using 31044-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 31044-1-AP (KHSRP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 31044-1-AP

  IP result of anti-KHSRP (IP:31044-1-AP, 4ug) with HeLa cells lysate 1440 ug; Detection: anti-KHSRP (WB: 31044-1-AP 1:8000) & anti-HNRNPC (WB: 11760-1-AP, 1:10000).

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  IHC staining of human colon using 31044-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 31044-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31044-1-AP (KHSRP antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31044-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (31044-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

31044-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA KHSRP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KHSRP fusion protein Ag33890
• Vollständiger Name: KH-type splicing regulatory protein
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 75-87kDa
• GenBank-Zugangsnummer: NM_003685
• Gene symbol: KHSRP
• Gene ID (NCBI): 8570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.