KHSRP Rekombinanter Antikörper

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KHSRP Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230189G1

Kat-Nr. : 82931-1-PBS

Synonyme

230189G1, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2

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  WB analysis using 82931-1-RR (same clone as 82931-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 82931-1-RR (same clone as 82931-1-PBS)

  WB result of KHSRP antibody (82931-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHSRP transfected HeLa cells. This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 82931-1-RR (same clone as 82931-1-PBS)

  IP result of anti-KHSRP (IP:82931-1-RR, 4ug; Detection:82931-1-RR 1:2500) with HeLa cells lysate 1050 ug. This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82931-1-RR (same clone as 82931-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82931-1-RR (KHSRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 82931-1-RR (same clone as 82931-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 82931-1-RR (same clone as 82931-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 82931-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82931-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82931-1-RR against Human KHSRP were performed. The affinity constant is 0.597 nM.

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Geprüfte Anwendungen

Produktinformation

82931-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA KHSRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KHSRP fusion protein Ag33890
• Vollständiger Name: KH-type splicing regulatory protein
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_003685
• Gene symbol: KHSRP
• Gene ID (NCBI): 8570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.