KHSRP Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

KHSRP Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230189G1

Kat-Nr. : 82931-1-RR

Synonyme

230189G1, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2

---

• 

  WB analysis of HeLa using 82931-1-RR

  WB result of KHSRP antibody (82931-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KHSRP transfected HeLa cells.

• 

  WB analysis using 82931-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82931-1-RR (KHSRP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 82931-1-RR

  IP result of anti-KHSRP (IP:82931-1-RR, 4ug; Detection:82931-1-RR 1:2500) with HeLa cells lysate 1050 ug.

• 

  IHC staining of mouse brain using 82931-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82931-1-RR (KHSRP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 82931-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KHSRP antibody (82931-1-RR, Clone: 230189G1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 82931-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug KHSRP Recombinant antibody (82931-1-RR, Clone:230189G1) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• 

  Affinity and Kinetic Characterization of 82931-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82931-1-RR against Human KHSRP were performed. The affinity constant is 0.597 nM.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, Jurkat-Zellen, Maushirngewebe, Mausmilzgewebe, Rattenhirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82931-1-RR bindet in WB, IHC, IF/ICC, FC (Intra), IP, ELISA KHSRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KHSRP fusion protein Ag33890
• Vollständiger Name: KH-type splicing regulatory protein
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_003685
• Gene symbol: KHSRP
• Gene ID (NCBI): 8570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possi3bly by recruiting degradation machinery to ARE-containing mRNAs.