KHSRP Rekombinanter Antikörper

KHSRP Rekombinant Antikörper für FC (Intra), IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC (Intra), IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

230189C12

Kat-Nr. : 82931-2-RR

Synonyme

230189C12, Far upstream element-binding protein 2, FBP2, FUBP2, FUSE-binding protein 2

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  IP experiment of HeLa using 82931-2-RR

  IP result of anti-KHSRP (IP:82931-2-RR, 4ug; Detection:82931-2-RR 1:2500) with HeLa cells lysate 1050 ug.

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  FC experiment of U-251 using 82931-2-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human KHSRP (82931-2-RR, Clone:230189C12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 82931-2-RR

  Biolayer interferometry (BLl) kinetic assays of 82931-2-RR against Human KHSRP were performed. The affinity constant is 1.42 nM.

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Geprüfte Anwendungen

• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): U-251-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82931-2-RR bindet in FC (Intra), IP, ELISA KHSRP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KHSRP fusion protein Ag33890
• Vollständiger Name: KH-type splicing regulatory protein
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_003685
• Gene symbol: KHSRP
• Gene ID (NCBI): 8570
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.