KHSRP Rekombinanter Antikörper

KHSRP Rekombinant Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

230189B2

Kat-Nr. : CL488-82931-3

Synonyme

KH type-splicing regulatory protein, FUSE-binding protein 2, FUBP2, FBP2, Far upstream element-binding protein 2

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  IF Staining of A549 using CL488-82931-3

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 KHSRP antibody (CL488-82931-3, Clone: 230189B2 ) at dilution of 1:200.

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  FC experiment of A549 using CL488-82931-3

  1x10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated KHSRP Recombinant antibody (CL488-82931-3, Clone:230189B2)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: A549-Zellen
• Erfolgreiche Detektion in FC (Intra): A549-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-82931-3 bindet in IF/ICC, FC (Intra) KHSRP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: KHSRP fusion protein Ag33890
• Vollständiger Name: KH-type splicing regulatory protein
• Berechnetes Molekulargewicht: 73 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_003685
• Gene symbol: KHSRP
• Gene ID (NCBI): 8570
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KHSRP, also named as FUBP2, KSRP, and p75, belongs to the KHSRP family. It binds to the dendritic targeting element and may play a role in mRNA trafficking. KHSRP is a part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. It mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. KHSRP may interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. It is involved in the degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.