Ki-67 Rekombinanter Antikörper

Ki-67 Rekombinant Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241499B1

Kat-Nr. : 84192-3-RR

Synonyme

KI67, MKI67, 241499B1, Antigen identified by monoclonal antibody Ki-67, Antigen KI-67

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• 

  WB analysis of MCF-7 using 84192-3-RR

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 84192-3-RR (Ki-67 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84192-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84192-3-RR (Ki-67 antibody) at dilution of 1:16000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovarian cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovarian cancer using 84192-3-RR

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 84192-3-RR (KI67 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human thyroid cancer using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human thyroid cancer tissue using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), ICAM-1 antibody (60299-1-Ig, Clone: 2F9A8, red). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of A549 using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of A431 using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of hTERT-RPE1 using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 84192-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KI67 antibody (84192-3-RR, Clone: 241499B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes malignes Melanomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe, humanes Schilddrüsenkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, A431-Zellen, A549-Zellen, HepG2-Zellen, hTERT-RPE1-Zellen, MCF-7-Zellen, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

84192-3-RR bindet in WB, IHC, IF/ICC, IF-P, ELISA Ki-67 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: antigen identified by monoclonal antibody Ki-67
• Berechnetes Molekulargewicht: 359 kDa
• GenBank-Zugangsnummer: NM_002417
• Gene symbol: KI67
• Gene ID (NCBI): 4288
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis.

Publikationen

Species	Application	Title
mouse	IHC	Ann Hematol; Efficacy and mechanism of the XPO1 inhibitor selinexor combined with decitabine in T-cell lymphoblastic lymphoma; Authors - Miaomiao Meng