KIAA0182 Polyklonaler Antikörper

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• KD/KO Validated

KIAA0182 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 24947-1-AP

Synonyme

Genetic suppressor element 1, GSE1, KIAA0182

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  WB analysis using 24947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24947-1-AP (KIAA0182 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 24947-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24947-1-AP (KIAA0182 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 24947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIAA0182 antibody (24947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 2 publications below

Produktinformation

24947-1-AP bindet in WB, IHC, IF/ICC, ELISA KIAA0182 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0182 fusion protein Ag20674
• Vollständiger Name: KIAA0182
• Berechnetes Molekulargewicht: 1217 aa, 136 kDa
• Beobachtetes Molekulargewicht: 136 kDa
• GenBank-Zugangsnummer: BC037556
• Gene symbol: KIAA0182
• Gene ID (NCBI): 23199
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
	WB	Cell Stem Cell; Engineering Genetic Predisposition in Human Neuroepithelial Stem Cells Recapitulates Medulloblastoma Tumorigenesis.; Authors - Miller Huang ; KO Validated
human	WB	Nat Commun; The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs; Authors - Andreas Herchenröther
human	WB	Sci Adv; Targeting the scaffolding role of LSD1 (KDM1A) poises acute myeloid leukemia cells for retinoic acid-induced differentiation.; Authors - Roberto Ravasio
human	WB	EMBO Mol Med; LSD1 inhibition induces differentiation and cell death in Merkel cell carcinoma.; Authors - Lukas Leiendecker
human	WB,IHC	J Biol Chem; GSE1 predicts poor survival outcome in gastric cancer patients by SLC7A5 enhancement of tumor growth and metastasis.; Authors - Keshuo Ding ; KD Validated
human	WB	Biochem Biophys Res Commun; GSE1 negative regulation by miR-489-5p promotes breast cancer cell proliferation and invasion.; Authors - Peng Chai