Rubicon Polyklonaler Antikörper

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• KD/KO Validated

Rubicon Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(22)

Rezensionen (1)

Kat-Nr. : 21444-1-AP

Synonyme

Baron, KIAA0226, RUBCN, Rubicon

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• 

  WB analysis of mouse liver using 21444-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21444-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 21444-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21444-1-AP (Rubicon antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 21444-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 21444-1-AP (Rubicon antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A431 using 21444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using Rubicon antibody (21444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 21444-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Rubicon antibody (21444-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Hodengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 18 publications below
• IHC: See 3 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Produktinformation

21444-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Rubicon und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Rubicon fusion protein Ag13715
• Vollständiger Name: KIAA0226
• Berechnetes Molekulargewicht: 972 aa, 109 kDa
• Beobachtetes Molekulargewicht: 110-130 kDa
• GenBank-Zugangsnummer: BC033615
• Gene symbol: Rubicon
• Gene ID (NCBI): 9711
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Rubicon (Run domain Beclin-1 interacting and cysteine-rich containing protein) consists of an N-terminal RUN domain, a middle region that includes its PI3K-binding domain (PIKBD), a coiled-coil domain (CCD), a serine-rich region (S-R), a helix-coil-rich domain (H-C) and a C-terminal Rubicon homology domain (RH) (PMID: 36288306). Rubicon is ubiquitously expressed in most tissue and organs.

Publikationen

Species	Application	Title
mouse	WB,IF	ACS Nano; Neutrophil Nanovesicle Protects against Experimental Autoimmune Encephalomyelitis through Enhancing Myelin Clearance by Microglia; Authors - Shishi Shen
human	WB,IF,IP	Adv Sci (Weinh); Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia; Authors - Tiemei Li
Escherichia coli	WB	Nat Commun; A mosquito salivary protein promotes flavivirus transmission by activation of autophagy.; Authors - Peng Sun
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
		Genes Dev; USP14 regulates autophagy by suppressing K63 ubiquitination of Beclin 1.; Authors - Daichao Xu
rat	WB,IF	Autophagy; RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.; Authors - Luis Muniz-Feliciano ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (07-21-2021)

The antibody seemed to work okay, although I had issues differentiating between autofluorescence and a positive signal. However, that is not the fault of the antibody, but rather my cells.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: iBMDMs