KIAA0319L Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

KIAA0319L Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(8)

Kat-Nr. : 21016-1-AP

Synonyme

PKD1 like, KIAA1837, KIAA0319 like, Dyslexia-associated protein KIAA0319-like protein, Adeno-associated virus receptor

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  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21016-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21016-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HuH-7-Zellen, MCF-7-Zellen, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, humanes Hirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Produktinformation

21016-1-AP bindet in WB, IHC, IF, ELISA KIAA0319L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0319L fusion protein Ag15263
• Vollständiger Name: KIAA0319-like
• Berechnetes Molekulargewicht: 1049 aa, 116 kDa
• Beobachtetes Molekulargewicht: ~150 kDa
• GenBank-Zugangsnummer: BC031672
• Gene symbol: KIAA0319L
• Gene ID (NCBI): 79932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).

Publikationen

Species	Application	Title
mouse	WB	Nat Methods; Hardwiring tissue-specific AAV transduction in mice through engineered receptor expression; Authors - James Zengel
human	WB	Sci Adv; Enhancement of liver-directed transgene expression at initial and repeat doses of AAV vectors admixed with ImmTOR nanoparticles.; Authors - Petr O Ilyinskii ; KD Validated
mouse	WB	Sci Rep; AU040320 deficiency leads to disruption of acrosome biogenesis and infertility in homozygous mutant mice.; Authors - Luiz G Guidi
human,mouse	IF	Gene Ther; Serotype survey of AAV gene delivery via subconjunctival injection in mice.; Authors - Liujiang Song
human	WB	Sci Rep; Correction of LAMP3-associated salivary gland hypofunction by aquaporin gene therapy; Authors - Hiroyuki Nakamura
human	WB	bioRxiv; Identification of SLC35A1 as an essential host factor for the transduction of multi-serotype recombinant adeno-associated virus (AAV) vectors; Authors - Xiujuan Zhang