KIAA0319L Polyklonaler Antikörper

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• KD/KO Validated

KIAA0319L Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21016-1-PBS

Synonyme

PKD1 like, KIAA1837, KIAA0319 like, Dyslexia-associated protein KIAA0319-like protein, Adeno-associated virus receptor

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  WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

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  WB analysis using 21016-1-AP (same clone as 21016-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21016-1-AP (KIAA0319L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 21016-1-AP (KIAA0319L antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 21016-1-AP (same clone as 21016-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21016-1-AP (KIAA0319L antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21016-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21016-1-PBS bindet in WB, IHC, Indirect ELISA KIAA0319L und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: KIAA0319L fusion protein Ag15263
• Vollständiger Name: KIAA0319-like
• Berechnetes Molekulargewicht: 1049 aa, 116 kDa
• Beobachtetes Molekulargewicht: ~150 kDa
• GenBank-Zugangsnummer: BC031672
• Gene symbol: KIAA0319L
• Gene ID (NCBI): 79932
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

KIAA0319L (also known as AAVR) is a type-I transmembrane protein that acts as a receptor for adeno-associated virus (AAV) and is involved in adeno-associated virus infection through endocytosis system (PMID: 26814968). It has been shown that KIAA0319L is highly-glycosylated and can localise to the plasma membrane, endosomes, Golgi and trans-Golgi network (TGN) (PMID: 29991750). KIAA0319L gene has been associated with susceptibility to developmental dyslexia or reading disabilities (PMID: 19085271). The predicted molecular mass of KIAA0319L is 116 kDa, but western blot detected protein at 150 kDa in lysate from HeLa cells, likely due to glycosidic linkages (PMID: 28679762; 30962536).