MRPP3 Polyklonaler Antikörper

MRPP3 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20959-1-AP

Synonyme

KIAA0391, Protein only RNase P catalytic subunit, PRORP, Mitochondrial ribonuclease P catalytic subunit, EC:3.1.26.5

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20959-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 20959-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20959-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20959-1-AP (MRPP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20959-1-AP

  Immunofluorescent analysis of HepG2 cells, using KIAA0391 antibody 20959-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20959-1-AP bindet in WB, IF/ICC, ELISA MRPP3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MRPP3 fusion protein Ag15196
• Vollständiger Name: KIAA0391
• Berechnetes Molekulargewicht: 583 aa, 67 kDa
• Beobachtetes Molekulargewicht: 65-67 kDa
• GenBank-Zugangsnummer: BC032221
• Gene symbol: MRPP3
• Gene ID (NCBI): 9692
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694)

Publikationen

Species	Application	Title
human	WB	Cell Metab; Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids.; Authors - Daniel F Bogenhagen